This post is also available in: Nederlands (Dutch) Français (French) polski (Polish) Español (Spanish)
The solution must be put in a specified format into the light path of a photometer for photometric examinations of liquid samples. The typical choice for this application is cuvettes, which are sample containers with optical windows.
Image source: science photo/shutterstock.com
The solution must be put in a specified format into the light path of a photometer for photometric examinations of liquid samples. The typical choice for this application is cuvettes, which are sample containers with optical windows. The spacing between the optical windows is precisely specified, allowing the sample’s route length inside the cuvette to be determined. Even if only those cuvettes utilized for absorbance measurements in the domain of UV-Vis spectroscopy are examined, the selection of different types of cuvettes is extensive. The square cuvette is the most common, having exterior dimensions of 12.5 x 12.5 mm. Sample quantities ranging from microliters (ultra-micro cuvettes) to milliliters (macro cuvettes) are accommodated in this format.
A cuvette’s typical path length is 10 mm, however there are also cuvettes that give a shorter light path across the sample. Furthermore, cuvettes differ in terms of material, height, and the size of the measuring window.