{"id":9944,"date":"2022-02-28T12:10:55","date_gmt":"2022-02-28T12:10:55","guid":{"rendered":"https:\/\/cotslab.com\/%ed%98%95%ea%b4%91-%ed%9d%a1%ea%b4%91%eb%8f%84%ec%97%90-%eb%8c%80%ed%95%9c-%ea%b0%80%ec%b9%98-%ec%9e%88%eb%8a%94-%ec%b6%94%ea%b0%80\/"},"modified":"2022-02-28T12:11:01","modified_gmt":"2022-02-28T12:11:01","slug":"%ed%98%95%ea%b4%91-%ed%9d%a1%ea%b4%91%eb%8f%84%ec%97%90-%eb%8c%80%ed%95%9c-%ea%b0%80%ec%b9%98-%ec%9e%88%eb%8a%94-%ec%b6%94%ea%b0%80","status":"publish","type":"post","link":"https:\/\/cotslab.com\/ko\/%ed%98%95%ea%b4%91-%ed%9d%a1%ea%b4%91%eb%8f%84%ec%97%90-%eb%8c%80%ed%95%9c-%ea%b0%80%ec%b9%98-%ec%9e%88%eb%8a%94-%ec%b6%94%ea%b0%80\/","title":{"rendered":"\ud615\uad11: \ud761\uad11\ub3c4\uc5d0 \ub300\ud55c \uac00\uce58 \uc788\ub294 \ucd94\uac00?"},"content":{"rendered":"\n

\ud575\uc0b0 \ubc0f \ub2e8\ubc31\uc9c8\uc758 \uc815\ub7c9\ud654\ub294 UV-Vis \ubd84\uad11\uad11\ub3c4\ubc95\uc744 \uc0ac\uc6a9\ud558\uc5ec \uc77c\uc0c1\uc801\uc73c\ub85c \uc218\ud589\ub429\ub2c8\ub2e4. \uadf8\ub7ec\ub098 \uacbd\uc6b0\uc5d0 \ub530\ub77c \ud615\uad11\uc744 \ud1b5\ud55c \uac80\ucd9c\uc774 \ub354 \uc720\ub9ac\ud55c \uac83\uc73c\ub85c \ud310\uba85\ub420 \uc218 \uc788\uc2b5\ub2c8\ub2e4. \uc774 \uacbd\uc6b0 \ubd84\uc790\ub294 \ud761\uad11\ub3c4\ub97c \ud1b5\ud574 \uac10\uc9c0\ub418\uc9c0 \uc54a\uc9c0\ub9cc \ud615\uad11 \uc5fc\ub8cc\uc758 \uc2e0\ud638\ub97c \ud1b5\ud574 \uac04\uc811\uc801\uc778 \ubc29\uc2dd\uc73c\ub85c \uac10\uc9c0\ub429\ub2c8\ub2e4. \ud615\uad11\uc740 \ub9e4\uc6b0 \ubbfc\uac10\ud55c \uac80\ucd9c \ubc29\ubc95\uc744 \uc81c\uacf5\ud560 \ubfd0\ub9cc \uc544\ub2c8\ub77c \uac1c\ubcc4 \ubd84\uc11d \ubb3c\uc9c8\uc744 \uc120\ud0dd\uc801\uc73c\ub85c \uce21\uc815\ud560 \uc218 \uc788\uc2b5\ub2c8\ub2e4.<\/p>\n\n

\uc0ac\ub840 1: \uc800\ub18d\ub3c4 \uc2dc\ub8cc \uc6a9\uc561\uc740 \uc5b4\ub5bb\uac8c \uc9c4\ud589\ud569\ub2c8\uae4c?<\/strong><\/p>\n\n

UV-Vis \ubd84\uad11\uad11\ub3c4\ubc95\uc740 \ub9e4\uc6b0 \ubbfc\uac10\ud558\uc9c0 \uc54a\uc2b5\ub2c8\ub2e4. \uc815\ud655\ud558\uace0 \uc815\ud655\ud55c \ub370\uc774\ud130\ub97c \uc5bb\uc73c\ub824\uba74 \uc0d8\ud50c \ub18d\ub3c4\uac00 \ud2b9\uc815 \ub18d\ub3c4 \uc784\uacc4\uac12 \uc544\ub798\ub85c \ub5a8\uc5b4\uc9c0\uc9c0 \uc54a\ub3c4\ub85d \ud574\uc57c \ud569\ub2c8\ub2e4. dsDNA\uc758 \uacbd\uc6b0 \uc774 \ud558\ud55c\uc740 \uc57d 1\u00b5g\/mL\uc785\ub2c8\ub2e4. \uad11\ub3c4\uacc4\uc758 \uac10\uc9c0 \ud558\ud55c\uc5d0 \ub3c4\ub2ec\ud558\uc9c0 \uc54a\uc740 \uacbd\uc6b0\uc5d0\ub3c4 \uc774 \ubc94\uc704\uc5d0\uc11c \uce21\uc815 \ubd80\uc815\ud655\ub3c4\uc758 \uc601\ud5a5\uc774 \ud06c\uba70 \uacb0\uacfc \uac12\uc774 \ud06c\uac8c \ubcc0\ud560 \uc218 \uc788\uc2b5\ub2c8\ub2e4. \ud761\uad11\ub3c4 \ubc29\ubc95\ubcf4\ub2e4 1000\ubc30 \ub354 \ubbfc\uac10\ud55c \ud615\uad11 \ubd84\uc11d\uc740 \uadf9\ub3c4\ub85c \ub0ae\uc740 \ub18d\ub3c4\uc758 \uc2dc\ub8cc \uc6a9\uc561\uc744 \ub9e4\uc6b0 \uc815\ud655\ud558\uac8c \uc815\ub7c9\ud654\ud560 \uc218 \uc788\uc2b5\ub2c8\ub2e4.<\/p>\n\n

\uc0ac\ub840 2: \uc0d8\ud50c \uc624\uc5fc\uc5d0\ub3c4 \ubd88\uad6c\ud558\uace0 \uc5b4\ub5bb\uac8c \uc815\ud655\ud55c \uc815\ub7c9\uc744 \uc218\ud589\ud560 \uc218 \uc788\uc2b5\ub2c8\uae4c?<\/strong><\/p>\n\n

260 nm\uc5d0\uc11c \ud575\uc0b0\uc758 \uc815\ub7c9\ud654 \uc678\uc5d0\ub3c4 UV-Vis \ubd84\uad11 \uad11\ub3c4\uacc4\ub294 \uac01\uac01 \ucd94\uac00 \ud30c\uc7a5(230 nm, 280 nm, 320 nm) \ub610\ub294 \ud30c\uc7a5 \uc2a4\uce94\uc744 \uc0ac\uc6a9\ud558\uc5ec \uc0d8\ud50c \uc21c\ub3c4\ub97c \uacb0\uc815\ud558\ub294 \uc635\uc158\uc744 \uc81c\uacf5\ud569\ub2c8\ub2e4. \ub370\uc774\ud130 \ubd84\uc11d\uc744 \ud1b5\ud574 \uc5fc\ubd84, \ub2e8\ubc31\uc9c8 \ub610\ub294 \uace0\uccb4 \uc785\uc790\uc640 \uac19\uc740 \ubb3c\uc9c8\uc758 \uc874\uc7ac\uc5d0 \uad00\ud55c \uacb0\ub860\uc744 \ub0b4\ub9b4 \uc218 \uc788\uc2b5\ub2c8\ub2e4. \ubc18\uba74\uc5d0 \ub2e4\ub978 \ud575\uc0b0\uc758 \uacbd\uc6b0\ucc98\ub7fc \ud761\uad11\ub3c4 \uc2a4\ud399\ud2b8\ub7fc\uc774 \ub108\ubb34 \uc720\uc0ac\ud55c \uacbd\uc6b0 \ud761\uad11\ub3c4\ub9cc\uc73c\ub85c\ub294 \uc608\ub97c \ub4e4\uc5b4 RNA\uc640 DNA\ub97c \uad6c\ubcc4\ud560 \uc218 \uc5c6\uc2b5\ub2c8\ub2e4. \uc774 \uacbd\uc6b0 \ud615\uad11 \uce21\uc815\uc740 \ub2e4\ub978 \ubd84\uc790\uc758 \uac04\uc12d \uc5c6\uc774 \ubd84\uc11d\ubb3c\uc758 \ud2b9\uc815 \uc815\ub7c9\ud654\ub97c \ud5c8\uc6a9\ud569\ub2c8\ub2e4.<\/p>\n\n

\uc774 \ubc29\ubc95\uc740 \uc5b4\ub5a4 \uc6d0\uce59\uc744 \uae30\ubc18\uc73c\ub85c \ud569\ub2c8\uae4c?<\/strong><\/p>\n\n

\ud615\uad11 \uce21\uc815\uc740 \ud2b9\uc815 \ubd84\uc790\uac00 \ube5b\uc758 \ud615\ud0dc\ub85c \uc5d0\ub108\uc9c0\ub97c \ubc29\ucd9c\ud560 \uc218 \uc788\ub2e4\ub294 \ud604\uc0c1\uc744 \uc774\uc6a9\ud569\ub2c8\ub2e4. \ud575\uc0b0 \ubc0f \ub2e8\ubc31\uc9c8\uacfc \uac19\uc740 \uc0dd\uccb4 \ubd84\uc790\uc758 \uac80\ucd9c\uc744 \uc704\ud574 \uac00\ub2a5\ud55c \uac00\uc7a5 \ub192\uc740 \ud2b9\uc774\ub3c4\ub85c \ubd84\uc11d\ubb3c\uc5d0 \uacb0\ud569\ud558\ub294 \ud615\uad11 \uc5fc\ub8cc\uac00 \uc120\ud0dd\ub429\ub2c8\ub2e4. \uc2dc\uc57d PicoGreen \u00ae<\/sup> \uacfc dsDNA\uc758 \uacb0\ud569\uc740 \uc804\ud615\uc801\uc778 \uc608\uc785\ub2c8\ub2e4. \ud2b9\uc815 \ud30c\uc7a5(PicoGreen\uc758 \uacbd\uc6b0 \uc57d 480nm)\uc758 \ube5b\uc744 \uc0ac\uc6a9\ud558\uc5ec \ubcf5\ud569\uccb4\ub97c \uc5ec\uae30\ud558\uba74 \ud615\uad11\ub2e8\uc740 \ub354 \ub0ae\uc740 \uc5d0\ub108\uc9c0, \uc989 \ub354 \uae34 \ud30c\uc7a5(PicoGreen\uc758 \uacbd\uc6b0 \uc57d 520nm)\uc758 \ube5b\uc744 \ubc29\ucd9c\ud569\ub2c8\ub2e4(\uadf8\ub9bc 1). \ube5b\uc740 \uc0c1\ub300 \ud615\uad11(RFU)\uc73c\ub85c \uce21\uc815\ub418\uba70, \uc5ec\uae30\uc11c \ud615\uad11\uc758 \uac15\ub3c4\ub294 \uc0d8\ud50c\uc758 \ub18d\ub3c4\uc5d0 \ube44\ub840\ud569\ub2c8\ub2e4.<\/p>\n\n

\"\"
\uadf8\ub9bc 1:\uac00) \ud575\uc0b0\uc758 \ud615\uad11 \uce21\uc815 \uc6d0\ub9ac(\uc88c)B) \ud615\uad11\ub2e8\uc744 \uc5ec\uae30\uc2dc\ud0a4\ub294 \ub370 \uc0ac\uc6a9\ub418\ub294 \ube5b(EX)\uc740 \ubc29\ucd9c\ub41c \ube5b(EM)\ubcf4\ub2e4 \ud30c\uc7a5\uc774 \uc9e7\uc2b5\ub2c8\ub2e4(\uc624\ub978\ucabd).<\/figcaption><\/figure><\/div>\n\n

\uc774 \ubc29\ubc95\uc5d0\ub294 \uc5b4\ub5a4 \uc7ac\ub8cc\uac00 \ud544\uc694\ud569\ub2c8\uae4c?<\/strong><\/p>\n\n

\ud575\uc0b0 \ubc0f \ub2e8\ubc31\uc9c8\uc758 \uc815\ub7c9\ud654\ub294 \ub192\uc740 \ud2b9\uc774\ub3c4\ub85c \uac01\uac01\uc758 \ud45c\uc801 \ubd84\uc790\uc5d0 \uacb0\ud569\ud558\ub294 \ud615\uad11 \uc5fc\ub8cc\ub97c \uc120\ud0dd\ud558\ub294 \uc774\uc810\uc774 \uc788\uc2b5\ub2c8\ub2e4. \ub610\ud55c \uc54c\ub824\uc9c4 \ub18d\ub3c4\uc758 \ud45c\uc900\uc774 \ud558\ub098 \uc774\uc0c1 \uc788\uc5b4\uc57c \ud569\ub2c8\ub2e4. \ud615\uad11\ub2e8\uc758 \uc5ec\uae30\uc640 \ubc29\ucd9c\ub41c \ube5b\uc758 \ud615\uad11 \uac15\ub3c4 \uce21\uc815\uc5d0\ub294 \ub3c5\ub9bd\ud615 \ud615\uad11\uacc4 \ub610\ub294 \ub2e4\ub978 \uae30\uae30\uc5d0 \ud1b5\ud569\ub41c \ud615\uad11 \ubaa8\ub4c8\uc774 \ud544\uc694\ud569\ub2c8\ub2e4. \uc7a5\ube44 \ub0b4\ubd80\uc758 \uad11\uc6d0\uc774 \ud544\uc694\ud55c \uc5ec\uae30 \ud30c\uc7a5\uc744 \uc81c\uacf5\ud558\uace0 \uac80\ucd9c\uae30\uac00 \ubc29\ucd9c\ub41c \ube5b\uc744 \uce21\uc815\ud560 \uc218 \uc788\ub3c4\ub85d \uc7a5\ube44\uc640 \ud615\uad11 \uc5fc\ub8cc\uac00 \ud638\ud658\ub418\uc5b4\uc57c \ud569\ub2c8\ub2e4. \uae30\uae30 \uc720\ud615\uc5d0 \ub530\ub77c \uc2dc\uc57d \uc6a9\uc561\uc740 \ubcbd\uc774 \uc587\uc740 \ubc18\uc751 \uc6a9\uae30 \ub610\ub294 \ud050\ubcb3\uc5d0 \uc788\uc2b5\ub2c8\ub2e4. \uc801\ud569\uc131, \ud22c\uba85\ub3c4, \uc790\ub3d9 \ud615\uad11\uc131 \ubc0f \ubd80\ud53c\uc640 \uad00\ub828\ud558\uc5ec \ud2b9\uc815 \uc751\uc6a9 \ud504\ub85c\uadf8\ub7a8\uacfc\uc758 \ud638\ud658\uc131\uc744 \uace0\ub824\ud574\uc57c \ud569\ub2c8\ub2e4.<\/p>\n\n

\ubc29\ubc95\uc740 \uc5b4\ub5bb\uac8c \uc218\ud589\ub429\ub2c8\uae4c?<\/strong><\/p>\n\n

\uadf8\ub9bc 2\ub294 \ud615\uad11 \uce21\uc815\uc758 \uc21c\uc11c\ub97c \ubcf4\uc5ec\uc90d\ub2c8\ub2e4. \ube14\ub7ad\ud06c, \ud45c\uc900 \ubc0f \uc0d8\ud50c\uc758 \uc900\ube44\ub294 \ud615\uad11 \uc5fc\ub8cc\ub97c \ucca8\uac00\ud55c \ud6c4 \uc9e7\uc740 \ubc30\uc591 \uae30\uac04\uc73c\ub85c \uc2dc\uc791\ub429\ub2c8\ub2e4. \uba3c\uc800, \ud45c\uc900 \uace1\uc120\uc744 \uc0dd\uc131\ud558\uae30 \uc704\ud574 \uc54c\ub824\uc9c4 \ub18d\ub3c4\uc758 \ud558\ub098 \uc774\uc0c1\uc758 \ud45c\uc900\uc774 \uce21\uc815\ub429\ub2c8\ub2e4. \uc0d8\ud50c \ub18d\ub3c4\uc758 \uacb0\uc815\uc740 \ub450 \ubc88\uc9f8 \ub2e8\uacc4\uc5d0\uc11c \uc774\ub8e8\uc5b4\uc9c0\uba70, \uc5ec\uae30\uc11c \uc0d8\ud50c\uc758 \uce21\uc815\ub41c \ud615\uad11 \uac15\ub3c4\ub294 \ud45c\uc900 \uace1\uc120\uacfc \uad00\ub828\ud558\uc5ec \ud3c9\uac00\ub429\ub2c8\ub2e4.<\/p>\n\n

\"\"
\uc6cc\ud06c\ud50c\ub85c \ud615\uad11<\/figcaption><\/figure><\/div>\n\n

\ud615\uad11 \u2013 \uc120\ud0dd \ubc29\ubc95?<\/strong><\/p>\n\n

\ud2b9\uc815 \uacbd\uc6b0\uc5d0 \ud615\uad11\ubd84\uc11d\ubc95\uc774 \uc774\uc810\uc744 \uc81c\uacf5\ud558\ub354\ub77c\ub3c4 \uace0\uc804\uc801\uc778 UV-Vis \ubd84\uad11\uad11\ub3c4\ubc95\uc740 \ud575\uc0b0 \ubc0f \ub2e8\ubc31\uc9c8\uc758 \uc815\ub7c9\ud654\uc640 \uad00\ub828\ud558\uc5ec \uacc4\uc18d\ud574\uc11c \ud45c\uc900 \ubc29\ubc95\uc785\ub2c8\ub2e4. \ubd84\uad11\uad11\ub3c4\ubc95\uc744 \ud1b5\ud574 \uc2dc\ub8cc \uc21c\ub3c4\ub97c \ud655\uc778\ud560 \uc218 \uc788\uc73c\uba70 \uace0\ub18d\ucd95 \uc6a9\uc561\uc744 \uc9c1\uc811 \ubd84\uc11d\ud560 \uc218 \uc788\uc2b5\ub2c8\ub2e4. \uc0d8\ud50c\uc758 \ud488\uc9c8\uacfc \ub2e4\uc6b4\uc2a4\ud2b8\ub9bc \uc560\ud50c\ub9ac\ucf00\uc774\uc158\uc758 \uc694\uad6c \uc0ac\ud56d\uc5d0 \ub530\ub77c \ub450 \uae30\uc220\uc744 \uacb0\ud569\ud558\ub294 \uac83\uc774 \uc88b\uc2b5\ub2c8\ub2e4. <\/p>\n\n

PicoGreen \u00ae<\/sup> \uc740 Molecular Probes, Inc.\uc758 \ub4f1\ub85d \uc0c1\ud45c\uc785\ub2c8\ub2e4. Corporation, Eugene, OR, USA. Eppendorf \u00ae<\/sup> , Eppendorf Brand Design, Eppendorf BioSpectrometer \u00ae<\/sup> , Uvette \u00ae<\/sup> \ubc0f Eppendorf \u00b5Cuvette \u00ae<\/sup> \ub294 \ub3c5\uc77c Eppendorf AG\uc758 \ub4f1\ub85d \uc0c1\ud45c\uc785\ub2c8\ub2e4. \uadf8\ub798\ud53d \ubc0f \uc774\ubbf8\uc9c0\ub97c \ud3ec\ud568\ud55c \ud310\uad8c \uc18c\uc720. \uc800\uc791\uad8c \u00a9 2019 Eppendorf AG.<\/em><\/p>\n\n

\uc6d0\ucc9c<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

\ud575\uc0b0 \ubc0f \ub2e8\ubc31\uc9c8\uc758 \uc815\ub7c9\ud654\ub294 UV-Vis \ubd84\uad11\uad11\ub3c4\ubc95\uc744 \uc0ac\uc6a9\ud558\uc5ec \uc77c\uc0c1\uc801\uc73c\ub85c \uc218\ud589\ub429\ub2c8\ub2e4. \uadf8\ub7ec\ub098 \uacbd\uc6b0\uc5d0 \ub530\ub77c \ud615\uad11\uc744 \ud1b5\ud55c \uac80\ucd9c\uc774 \ub354 \uc720\ub9ac\ud55c \uac83\uc73c\ub85c \ud310\uba85\ub420 \uc218 \uc788\uc2b5\ub2c8\ub2e4. \uc774 \uacbd\uc6b0 \ubd84\uc790\ub294 \ud761\uad11\ub3c4\ub97c \ud1b5\ud574 \uac10\uc9c0\ub418\uc9c0 \uc54a\uc9c0\ub9cc \ud615\uad11 \uc5fc\ub8cc\uc758 \uc2e0\ud638\ub97c \ud1b5\ud574 \uac04\uc811\uc801\uc778 \ubc29\uc2dd\uc73c\ub85c \uac10\uc9c0\ub429\ub2c8\ub2e4. \ud615\uad11\uc740 \ub9e4\uc6b0 \ubbfc\uac10\ud55c \uac80\ucd9c \ubc29\ubc95\uc744 \uc81c\uacf5\ud560 \ubfd0\ub9cc \uc544\ub2c8\ub77c \uac1c\ubcc4 \ubd84\uc11d \ubb3c\uc9c8\uc744 \uc120\ud0dd\uc801\uc73c\ub85c \uce21\uc815\ud560 \uc218 \uc788\uc2b5\ub2c8\ub2e4. \uc0ac\ub840 1: \uc800\ub18d\ub3c4 \uc2dc\ub8cc \uc6a9\uc561\uc740 […]\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[493],"tags":[],"_links":{"self":[{"href":"https:\/\/cotslab.com\/ko\/wp-json\/wp\/v2\/posts\/9944"}],"collection":[{"href":"https:\/\/cotslab.com\/ko\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/cotslab.com\/ko\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/cotslab.com\/ko\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/cotslab.com\/ko\/wp-json\/wp\/v2\/comments?post=9944"}],"version-history":[{"count":1,"href":"https:\/\/cotslab.com\/ko\/wp-json\/wp\/v2\/posts\/9944\/revisions"}],"predecessor-version":[{"id":9958,"href":"https:\/\/cotslab.com\/ko\/wp-json\/wp\/v2\/posts\/9944\/revisions\/9958"}],"wp:attachment":[{"href":"https:\/\/cotslab.com\/ko\/wp-json\/wp\/v2\/media?parent=9944"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/cotslab.com\/ko\/wp-json\/wp\/v2\/categories?post=9944"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/cotslab.com\/ko\/wp-json\/wp\/v2\/tags?post=9944"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}