{"id":9808,"date":"2022-02-28T11:25:55","date_gmt":"2022-02-28T11:25:55","guid":{"rendered":"https:\/\/cotslab.com\/speictreafotaimeadracht-uv-vis-aigead-nuicleach-a-chainniochtu-easca-agus-thapa\/"},"modified":"2022-02-28T11:25:58","modified_gmt":"2022-02-28T11:25:58","slug":"speictreafotaimeadracht-uv-vis-aigead-nuicleach-a-chainniochtu-easca-agus-thapa","status":"publish","type":"post","link":"https:\/\/cotslab.com\/ga\/speictreafotaimeadracht-uv-vis-aigead-nuicleach-a-chainniochtu-easca-agus-thapa\/","title":{"rendered":"Speictreaf\u00f3taim\u00e9adracht UV-Vis \u2013 Aig\u00e9ad N\u00faicl\u00e9ach a Chainn\u00edocht\u00fa \u00c9asca agus Thapa"},"content":{"rendered":"\n<p>Is leor tomhas ions\u00fach\u00e1in tuaslag\u00e1in aig\u00e9id n\u00faicl\u00e9igh ag ceithre thonnfhad amh\u00e1in chun a thi\u00fachan a chinneadh agus chun l\u00e9argas a fh\u00e1il ar a \u00edonacht.<\/p>\n\n<p><em>Foinse \u00edomh\u00e1: DRogatnev\/shutterstock.com<\/em><\/p>\n\n<p>D\u00e9antar aig\u00e9id n\u00faicl\u00e9acha a leithlisi\u00fa \u00f3 \u00e1bhar samplach ar n\u00f3s cealla agus \u00fas\u00e1idtear iad ina dhiaidh sin i dturgnaimh saotharlainne breise. Chun na cr\u00edche sin, t\u00e1 s\u00e9 inmholta ti\u00fachan an DNA \u00edonaithe n\u00f3 RNA a chinneadh chomh maith lena n-\u00edonacht a fh\u00edor\u00fa. Ar an mbealach seo, cuirfidh m\u00e9ideanna at\u00e1 sainithe go cruinn an aig\u00e9id n\u00faicl\u00e9igh isteach san iarratas le sruth, agus aims\u00edtear aon \u00e9illi\u00fach\u00e1in a d&#8217;fh\u00e9adfadh tionchar a bheith acu ar imoibri\u00fa \u00edogair n\u00f3 ar mheas\u00fan\u00fa i bhf\u00edor-am.<\/p>\n\n<p>Is modh \u00e9 speictrophotim\u00e9adracht UV-Vis at\u00e1 \u00e9asca, gasta agus a nd\u00e9antar t\u00e1st\u00e1il ama air chun na cusp\u00f3ir\u00ed seo a bhaint amach. Sa raon 260 nm, l\u00e9ir\u00edonn aig\u00e9id n\u00faicl\u00e9acha buaic tr\u00e9ith ions\u00fach\u00e1in (figi\u00far 1). \u00das\u00e1idtear an luach ions\u00fach\u00e1in seo mar sin chun ti\u00fachain aig\u00e9id n\u00faicl\u00e9igh a r\u00edomh. De r\u00e9ir dhl\u00ed Lambert-Beer, t\u00e1 g\u00e1 le dh\u00e1 pharaim\u00e9adar chun ti\u00fachan an tsampla a r\u00edomh: fad an chos\u00e1in opt\u00fail (= fad cos\u00e1n solais (L)) agus comh\u00e9ifeacht d\u00edothaithe molar (tairiseach ar leith \u00e1bhar agus tonnfhad) den sampla le tomhas. Is f\u00e9idir an facht\u00f3ir sonrach (F) a r\u00edomh nuair a \u00fas\u00e1idtear cuvette caighde\u00e1nach le cos\u00e1n solais 1 cm. D\u00e9antar an facht\u00f3ir seo a iolr\u00fa ansin faoin luach ions\u00faite tomhaiste (A) chun ti\u00fachan (C) an tuaslag\u00e1in samplach a bhaint amach. Is gn\u00e1ch go mb\u00edonn comh\u00e9ifeachta\u00ed d\u00edothaithe m\u00f3lar agus facht\u00f3ir\u00ed sonracha, faoi seach, ar f\u00e1il sa litr\u00edocht. Is \u00e9 an facht\u00f3ir do dsDNA, mar shampla, n\u00e1 50 \u00b5g\/mL (RNA: 40 \u00b5g\/mL), agus de r\u00e9ir sainmh\u00ednithe is ionann \u00e9 agus aonad ions\u00fach\u00e1in amh\u00e1in. na sonra\u00ed seo.<\/p>\n\n<figure class=\"wp-block-table\"><table><tbody><tr><td><strong>Dl\u00ed Lambert-Beoir:<\/strong><br\/><br\/> <strong>C = A\/(L x \u0190 )<\/strong><br\/> <strong>F = 1\/\u0190<\/strong> (le haghaidh cos\u00e1n solais 1 cm)<br\/><br\/>  <strong> &#8211; C = A x F<\/strong><\/td><td> C = Ti\u00fachan<br\/> A = Ions\u00fa<br\/> L = Fad cos\u00e1in opt\u00fala (\u00e9adrom).<br\/> \u0190 = Comh\u00e9ifeacht d\u00edothaithe molar<br\/> (sampla agus tonnfhad-shonrach)<br\/> F = facht\u00f3ir<\/td><\/tr><\/tbody><\/table><\/figure>\n\n<div class=\"wp-block-image\"><figure class=\"aligncenter size-large\"><img loading=\"lazy\" width=\"768\" height=\"456\" src=\"https:\/\/cotslab.com\/wp-content\/uploads\/2021\/10\/csm_Figure_One_Rahmen_b55fd8fbd6.jpg\" alt=\"\" class=\"wp-image-9813\" srcset=\"https:\/\/cotslab.com\/wp-content\/uploads\/2021\/10\/csm_Figure_One_Rahmen_b55fd8fbd6.jpg 768w, https:\/\/cotslab.com\/wp-content\/uploads\/2021\/10\/csm_Figure_One_Rahmen_b55fd8fbd6-674x400.jpg 674w, https:\/\/cotslab.com\/wp-content\/uploads\/2021\/10\/csm_Figure_One_Rahmen_b55fd8fbd6-600x356.jpg 600w\" sizes=\"(max-width: 768px) 100vw, 768px\" \/><figcaption>F\u00edor 1: Speictream ions\u00fach\u00e1in aig\u00e9id n\u00faicl\u00e9acha le tonnfhaid \u00e1bhartha agus sampla ag cur s\u00edos ar r\u00edomh ti\u00fachan sampla dsDNA.<\/figcaption><\/figure><\/div>\n\n<p>Chun a bheith in ann r\u00e1iteas a dh\u00e9anamh faoi \u00edonacht sampla aig\u00e9id n\u00faicl\u00e9igh, n\u00ed m\u00f3r an t-ions\u00fachtadh ag tonnfhaid seachas 260 nm a chinneadh (figi\u00far 1). Is ionann na comhrann\u00e1in a dh\u00edortha\u00edtear \u00f3 na luachanna ions\u00fach\u00e1in arna dtomhas ag 260 nm, 280 nm agus 230 nm (A <sub>260<\/sub> \/A <sub>280<\/sub> agus A <sub>260<\/sub> \/A <sub>230<\/sub> ) agus na c\u00f3imheasa \u00edonachta a chabhra\u00edonn le h\u00e9illi\u00fach\u00e1in fh\u00e9ideartha a aithint. Tugann neamh\u00edonachta\u00ed cos\u00fail le pr\u00f3it\u00e9in\u00ed agus rianta imoibrithe a \u00fas\u00e1ideadh le linn an phr\u00f3isis \u00edon\u00fach\u00e1in speictream ions\u00fach\u00e1in difri\u00fail \u00f3 speictream aig\u00e9id n\u00faicl\u00e9acha agus mar sin b\u00edonn tionchar acu ar na c\u00f3imheasa \u00edonachta (figi\u00far 2). Beidh an c\u00f3imheas A <sub>260<\/sub> \/A <sub>280<\/sub> de thuaslag\u00e1in aig\u00e9id n\u00faicl\u00e9igh \u00edon thart ar 1.8 &#8211; 2.0, ach go hiond\u00fail l\u00e9ireoidh an c\u00f3imheas A <sub>260<\/sub> \/A <sub>230<\/sub> luachanna sa raon 2.0 \u2013 2.5.<\/p>\n\n<figure class=\"wp-block-image size-large\"><img loading=\"lazy\" width=\"768\" height=\"445\" src=\"https:\/\/cotslab.com\/wp-content\/uploads\/2021\/10\/csm_Figure_Two_Applications_389bfe613c.jpg\" alt=\"\" class=\"wp-image-9816\" srcset=\"https:\/\/cotslab.com\/wp-content\/uploads\/2021\/10\/csm_Figure_Two_Applications_389bfe613c.jpg 768w, https:\/\/cotslab.com\/wp-content\/uploads\/2021\/10\/csm_Figure_Two_Applications_389bfe613c-690x400.jpg 690w, https:\/\/cotslab.com\/wp-content\/uploads\/2021\/10\/csm_Figure_Two_Applications_389bfe613c-600x348.jpg 600w\" sizes=\"(max-width: 768px) 100vw, 768px\" \/><figcaption>F\u00edor 2: Speictrim ions\u00fach\u00e1in aig\u00e9id n\u00faicl\u00e9acha agus \u00e1bhar salaithe f\u00e9ideartha<\/figcaption><\/figure>\n\n<p>\u00das\u00e1idtear ceathr\u00fa tonnfhad chun an c\u00falra a chinneadh. D\u00e9antar \u00e9 a thomhas ag 320 nm n\u00f3 os a chionn toisc nach n-ions\u00fann aig\u00e9id n\u00faicl\u00e9acha n\u00e1 \u00e1bhair shalaithe org\u00e1nacha solas ag an tonnfhad seo. D\u2019fh\u00e9adfadh go l\u00e9ireodh ions\u00fa a thomhaistear sa raon seo l\u00e1ithreacht c\u00e1ithn\u00edn\u00ed n\u00f3 boilgeoga aeir laistigh den sampla. T\u00e1 fi\u00fa cuvette smudged in ann l\u00e9amh c\u00falra a mhealladh. Tugann f\u00f3taim\u00e9adair nua-aimseartha an rogha chun gn\u00e9 ceart\u00fach\u00e1in c\u00falra a ghn\u00edomhacht\u00fa a chuirfidh i bhfeidhm aon ions\u00fa c\u00falra \u00f3 gach luach tomhaiste eile a dheal\u00fa go huathoibr\u00edoch.<\/p>\n\n<p>Ina theannta sin, f\u00e9adfar speictream ioml\u00e1n sampla aig\u00e9id n\u00faicl\u00e9igh a ghabh\u00e1il (go hiond\u00fail idir 220 agus 320 nm). Tr\u00ed chompar\u00e1id\u00ed a dh\u00e9anamh leis an speictream a fhaightear \u00f3 thuaslag\u00e1n d&#8217;aig\u00e9ad n\u00faicl\u00e9ach \u00edon beifear in ann earr\u00e1id\u00ed a d&#8217;fh\u00e9adfadh a bheith ann a bhrath le linn an phr\u00f3isis tomhais chomh maith le l\u00e1ithreacht \u00e1bhar salaithe laistigh den sampla.<\/p>\n\n<p><a href=\"http:\/\/handling-solutions.eppendorf.com\/\">Foinse<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Is leor tomhas ions\u00fach\u00e1in tuaslag\u00e1in aig\u00e9id n\u00faicl\u00e9igh ag ceithre thonnfhad amh\u00e1in chun a thi\u00fachan a chinneadh agus chun l\u00e9argas a fh\u00e1il ar a \u00edonacht. Foinse \u00edomh\u00e1: DRogatnev\/shutterstock.com D\u00e9antar aig\u00e9id n\u00faicl\u00e9acha a leithlisi\u00fa \u00f3 \u00e1bhar samplach ar n\u00f3s cealla agus \u00fas\u00e1idtear iad ina dhiaidh sin i dturgnaimh saotharlainne breise. Chun na cr\u00edche sin, t\u00e1 s\u00e9 inmholta [&#8230;]\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[489],"tags":[],"_links":{"self":[{"href":"https:\/\/cotslab.com\/ga\/wp-json\/wp\/v2\/posts\/9808"}],"collection":[{"href":"https:\/\/cotslab.com\/ga\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/cotslab.com\/ga\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/cotslab.com\/ga\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/cotslab.com\/ga\/wp-json\/wp\/v2\/comments?post=9808"}],"version-history":[{"count":1,"href":"https:\/\/cotslab.com\/ga\/wp-json\/wp\/v2\/posts\/9808\/revisions"}],"predecessor-version":[{"id":9820,"href":"https:\/\/cotslab.com\/ga\/wp-json\/wp\/v2\/posts\/9808\/revisions\/9820"}],"wp:attachment":[{"href":"https:\/\/cotslab.com\/ga\/wp-json\/wp\/v2\/media?parent=9808"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/cotslab.com\/ga\/wp-json\/wp\/v2\/categories?post=9808"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/cotslab.com\/ga\/wp-json\/wp\/v2\/tags?post=9808"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}