Category Archives: Spectrophotometry

UV-Vis Spectrophotometry – Easy and Quick Quantification of Nucleic Acids

The absorbance measurement of a nucleic acid solution at only four wavelengths is sufficient to determine its concentration and to obtain an insight into its purity. Image source: DRogatnev/ Nucleic acids are isolated from sample material such as cells and subsequently employed in further laboratory experiments. For this purpose, it is advisable to determine the […]

Fluorescence: A Valuable Addition to Absorbance?

Quantification of nucleic acids and proteins is routinely performed using UV-Vis spectrophotometry; however, on occasion, detection via fluorescence may prove to be more favorable. In this case, molecules are not detected through absorbance, but they are detected in an indirect fashion – via the signal of a fluorescent dye. Not only does fluorescence offer a […]