Explained: UV vis Spectrophotometer and Fluorescence Cuvettes

In This Article: Introduction How do you define a cuvette? Understanding Cuvette Path Length Types of Cuvette Materials Cuvette Chemical Resistance Understanding Cuvette Volumes What is the Cuvette Z dimension, Exactly? Cuvette Caps   Introduction Any material that is transparent to the required wavelengths can be used to make a cuvette. And a wide selection […]

What’s the Difference Between a Quartz and a Glass Cuvette?

Let’s begin by considering the following scenario for a moment: It’s time to start a new lab job. New methods, new coworkers, new equipment, and, most importantly, new glass cuvettes! But wait, these cells aren’t labeled… Is this a quartz cuvette or a glass material? We receive calls like this all the time from individuals […]

UV-Vis Spectrophotometry – Easy and Quick Quantification of Nucleic Acids

The absorbance measurement of a nucleic acid solution at only four wavelengths is sufficient to determine its concentration and to obtain an insight into its purity. Image source: DRogatnev/shutterstock.com Nucleic acids are isolated from sample material such as cells and subsequently employed in further laboratory experiments. For this purpose, it is advisable to determine the […]

Fluorescence: A Valuable Addition to Absorbance?

Quantification of nucleic acids and proteins is routinely performed using UV-Vis spectrophotometry; however, on occasion, detection via fluorescence may prove to be more favorable. In this case, molecules are not detected through absorbance, but they are detected in an indirect fashion – via the signal of a fluorescent dye. Not only does fluorescence offer a […]